International Standard 22412:2008 Particle size analysis dynamic light scattering (DLS). Sample preparation either by filtration or centrifugation is critical to remove dust and artifacts from the solution. Experimental data using colloidal gold highlights the differences in the results that might be obtained with DLS compared to electron microscopy and how this can be used to gain a better understanding of the nanoparticle system. This scattered light then undergoes either constructive or destructive interference by the surrounding particles, and within this intensity fluctuation, information is contained about the time scale of movement of the scatterers. This fluctuation is due to the fact that the small molecules in solutions are undergoing Brownian motion, and so the distance between the scatterers in the solution is constantly changing with time. If the light source is a laser, and thus is monochromatic and coherent, the scattering intensity fluctuates over time. When light hits small particles, the light scatters in all directions ( Rayleigh scattering) as long as the particles are small compared to the wavelength (below 250 nm). In vertical/horizontal (VH) geometry the second polarizer allows light not in same direction as the incident light. the surfactant is a common sodium oleate, which has a CMC of 1. One is a vertical/vertical (VV) geometry, where the second polarizer allows light through that is in the same direction as the primary polarizer. Im doing research about how the concentration impacts the micelles size by DLS (dynamic light scattering). The data of nanoparticle dynamic light scattering (DLS) were analyzed in the mixed program of MATLAB and LabVIEW, where the autocorrelation functions of. From there the HDD and the polydispersity index (PDI) are calculated. Figure 1: Exemplary correlation function of a DLS measurement. The polarizers can be set up in two geometrical configurations. Dynamic light scattering (DLS) results DLS measurements provide results as the hydrodynamic diameter (HDD) and peak size (see Figure 1 and 2). This process is repeated at short time intervals and the resulting set of speckle patterns are analyzed by an autocorrelator that compares the intensity of light at each spot over time. The diffracted light from all of the molecules can either interfere constructively (light regions) or destructively (dark regions). To obtain higher quality DLS data use a protein concentration. All of the molecules in the solution are being hit with the light and all of the molecules diffract the light in all directions. dynamic light scattering and solubility analysis in the center of the process the chances.